Journal: bioRxiv

Article Title: Ion channel expression and electrophysiology of singular human (primary and induced pluripotent stem cell derived) cardiomyocytes

doi: 10.1101/2021.03.04.433834

Figure Lengend Snippet: Data are shown as mean ± SD. Students t-test was used to compare groups. * indicates p<0.05; ** indicates p<0.01, and *** indicates p<0.001. The numbers of total recordings used to calculate means are listed in . Note that electrophysiological recordings were not available from primary atrial cardiomyocytes.

Article Snippet: The procurement of donor hearts and the isolation of primary human cardiomyocytes was performed at AnaBios Corporation (San Diego, CA, USA).

Techniques:

Journal: bioRxiv

Article Title: Ion channel expression and electrophysiology of singular human (primary and induced pluripotent stem cell derived) cardiomyocytes

doi: 10.1101/2021.03.04.433834

Figure Lengend Snippet: To display the spectrum of action potential durations, a cell with a long and a cell with a short action potential for each cell group ( (a) iCell cardiomyocytes, (b) ventricular and (c) atrial Pluricytes, (d) primary adult human ventricular cardiomyocytes) was selected. A short action potential is shown in green and a long action potential is shown in red. The numbers of total recordings are listed in . Note that electrophysiological recordings were not available from primary atrial cardiomyocytes.

Article Snippet: The procurement of donor hearts and the isolation of primary human cardiomyocytes was performed at AnaBios Corporation (San Diego, CA, USA).

Techniques:

Journal: bioRxiv

Article Title: Ion channel expression and electrophysiology of singular human (primary and induced pluripotent stem cell derived) cardiomyocytes

doi: 10.1101/2021.03.04.433834

Figure Lengend Snippet: Evaluation of histograms to confirm cardiac subpopulations. In (a) the separated data sets of primary atrial and ventricular cardiomyocytes clearly show the expected left-shifted distribution of atrial cardiomyocytes (higher expression of this atrial-associated gene). However, the histogram of the pooled data sets in (b) demonstrates that differences between atrial and ventricular primary cardiomyocytes are too small to be resolved (for our number of investigated cells). Thus, it is unlikely that histograms of expression data from iCell cardiomyocytes (c) are suitable to confirm three subpopulations. For total n see . The histograms of the other ion channels investigated can be found in Supp .

Article Snippet: The procurement of donor hearts and the isolation of primary human cardiomyocytes was performed at AnaBios Corporation (San Diego, CA, USA).

Techniques: Expressing

Journal: bioRxiv

Article Title: Ion channel expression and electrophysiology of singular human (primary and induced pluripotent stem cell derived) cardiomyocytes

doi: 10.1101/2021.03.04.433834

Figure Lengend Snippet: As data of electrophysiological parameters are only available for primary ventricular cardiomyocytes (and not for primary atrial cardiomyocytes), we cannot evaluate whether shapes of histograms of electrophysiological parameters mirror cardiac subpopulations. However, the histograms of electrophysiological data from iCell cardiomyoctes do not indicate subpopulations. The total range of values is bigger than for primary ventricular cardiomyocytes (except for capacitance). For total n see .

Article Snippet: The procurement of donor hearts and the isolation of primary human cardiomyocytes was performed at AnaBios Corporation (San Diego, CA, USA).

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: LncRNA NKILA was upregulated in diabetic cardiomyopathy with early prediction values

doi: 10.3892/etm.2019.7671

Figure Lengend Snippet: Expression of lncRNA NKILA in cardiomyocyte cells is not affected by high-glucose treatment. LncRNA NKILA, nuclear factor-κB interacting long non-coding RNA.

Article Snippet: Primary human cardiomyocyte cells (T4037; Applied Biological Materials) were cultured in this study to perform in vitro cell experiments under conditions as recommend by the manufacturer.

Techniques: Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: LncRNA NKILA was upregulated in diabetic cardiomyopathy with early prediction values

doi: 10.3892/etm.2019.7671

Figure Lengend Snippet: LncRNA NKILA promotes apoptosis of cardiomyocytes. (A) Ectopic expression of the lncRNA NKILA vector doubled the levels of lncRNA NKILA mRNA and knockdown rate reached 50% at 24 h after transfection. (B) LncRNA NKILA overexpression accelerated, while lncRNA NKILA knockdown inhibited the apoptosis of cardiomyocytes, respectively. *P<0.05 vs. C and NC. LncRNA NKILA, nuclear factor-κB interacting long non-coding RNA; C, control; NC, negative control; siRNA, small interfering RNA.

Article Snippet: Primary human cardiomyocyte cells (T4037; Applied Biological Materials) were cultured in this study to perform in vitro cell experiments under conditions as recommend by the manufacturer.

Techniques: Expressing, Plasmid Preparation, Knockdown, Transfection, Over Expression, Control, Negative Control, Small Interfering RNA